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Web12 Apr 2024 · Perform PCR using a thermal cycler with a heated lid and following cycle conditions: 5 min at 95 °C, then 32 cycles of 15 s at 95 °C, 25 s at 57 °C, 15 s at 72 °C, then 2 min at 72 °C, followed by 16 °C hold. 5. Purify the PCR product (136 bps) after electrophoresis on 2% agarose gel with the gel purification kit (see Note 15). 6. WebTP350 User’s Manual Chapter 7: Specification (*)Performance specification apply when system is operated in the 20 to 23 degree C temperature range. Product name TaKaRa … ramblers live stream
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